Abcam, ab300636, Anti-AF6 antibody [35/AF6] - BSA and Azide Free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal I Afadin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:35/AF6 H1L1,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab300636 is a carrier free version of
ab300635
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AF6 also known as Afadin or anti-i is a protein involved in cell signaling and adhesion mechanisms. It weighs approximately 180 kDa and plays a role in the formation of tight junctions influencing cellular architecture and communication. AF6 is expressed in a variety of tissues including epithelial cells indicating its involvement in maintaining tissue integrity. The protein localizes at cellular junctions where it interacts with other junctional components facilitating connections between adjacent cells.
Biological function summary
AF6 contributes to cell-to-cell contact by organizing cytoskeletal elements and signaling elements. It exists within the nectin-afadin complex which is critical for the regulation of tight junctions and adherens junctions. This positioning allows AF6 to modulate processes like cell migration and tissue morphogenesis. Through its interactions with actin filaments and other junctional proteins AF6 has a significant role in maintaining proper cellular organization and stability.
Pathways
AF6 participates actively in the Ras signaling and Rap1 pathways. Within the Ras pathway AF6 associates with proteins like Ras and Rap1 which regulate various cellular outcomes including proliferation and differentiation. AF6's involvement with these pathways enhances its role in influencing cell dynamics and signaling decisions. AF6's function in these signaling pathways underlines its participation in normal cell function and development.
AF6 has links to cancer progression and neurological disorders such as autism. The protein’s interaction with Ras influences oncogenic signaling which can lead to aberrant cell growth and metastasis in cancers. Additionally disruptions in AF6 function or expression levels have associations with autism spectrum disorders as AF6 affects neural cell adhesion and signaling pathways. The protein’s interactions with junctional proteins like cadherins and nectins in diseased states suggest its potential as a therapeutic target.