Abcam, ab300648, Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free

Size: 100µg / 1mg
Rat Recombinant Monoclonal MYH10 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:EPR22564-23,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, IP, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab300648 is a carrier free version of
ab300647
This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (
ab230823
). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography DEAE-C, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Non-muscle Myosin IIB also known as MYH10 is a motor protein important in cell division and movement. This protein weighs approximately 228 kDa. Myosin IIB shows expression in various tissues including the brain heart and fibroblasts. Unlike other myosins found in muscle cells Myosin IIB functions mainly in non-muscle cells where it plays a role in organizing intracellular structure and shape.
Biological function summary
The non-muscle Myosin IIB contributes to cell motility and cytokinesis important for normal cell proliferation and maintenance. It operates as part of the actomyosin complex that includes actin and other myosin types. This complex facilitates contractile forces within cells enabling movements such as cell migration and tissue development. Myosin IIB works closely with actin filaments converting chemical energy from ATP into mechanical work required for these processes.
Pathways
Cell migration and tissue morphogenesis involve Myosin IIB. Myosin IIB participates in the Rho-ROCK signaling pathway which regulates actomyosin contraction during cellular movement and morphogenesis. It interacts with other myosins like Myosin IIA illustrating its role in shared pathways. These pathways control the balance between cellular adhesion and detachment necessary for proper cellular function.
Non-muscle Myosin IIB associates with neurological disorders and cancer. Changes in Myosin IIB function can disrupt normal cell division leading to cancer progression. In neurological disorders Myosin IIB affects cell signaling and structural integrity of neurons. For instance disruptions in interactions with actin and other related proteins like Myosin IIA and Tropomyosins can lead to these adverse biological effects showing its significance in maintaining healthy cellular operations.