Abcam, ab300724, Anti-Calcineurin B/PPP3R1+Calcineurin BII/PPP3R2 antibody [EPR24992-14] -BSA & Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24992-14,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Mouse, Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab300724 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human Calcineurin B/CNB. The recommended pair for sandwich ELISA is:
Capture:
ab200723
, human Calcineurin B/CNB Capture Antibody (unconjugated)
Detector: ab300724, human Calcineurin B/CNB Detector Antibody (unconjugated)
The reference range value is 0.16-20 ng/mL.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calcineurin B is a regulatory subunit of the enzyme calcineurin known alternatively as PPP3R1 for Calcineurin BI and PPP3R2 for Calcineurin BII. This protein weighs approximately 19 kDa. Calcineurin B is expressed in various tissues including brain muscle and immune cells. Its primary function is to bind calcium ions facilitating conformational changes that enable the activation of the catalytic subunit of calcineurin. Through these changes it maintains a connection between calcium signaling and other cellular processes.
Biological function summary
Calcineurin B plays an essential role in intracellular signaling by being part of the calcineurin complex which also includes the catalytic subunit calcineurin A. This complex is vital for the dephosphorylation of the nuclear factor of activated T-cells (NFAT) which then translocates to the nucleus to influence the expression of various genes. By controlling gene transcription calcineurin B contributes significantly to immune response muscle development and brain function.
Pathways
Calcineurin B facilitates various cellular reactions as an integral component of calcium signaling and immune response. It is significantly involved in the T-cell receptor signaling pathway where its activity helps regulate T-cell activation and differentiation. Calcineurin B also plays a role in the synaptic plasticity pathway important for learning and memory functions in the brain. It interacts closely with proteins like NFATc and calmodulin that further extend its influence across other signaling pathways.
Calcineurin B is implicated in conditions such as autoimmune diseases and cardiac hypertrophy. In autoimmune diseases disrupted calcineurin function can lead to improper T-cell activity contributing to diseases like rheumatoid arthritis. In cardiac hypertrophy altered calcineurin signaling can affect heart muscle growth and function. Calcineurin B is connected to the protein FKBP12 which is part of the immunosuppressive drug complex influencing its role in these disorders.