Abcam, ab301240, Human SLC2A3 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC2A3 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC2A3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucose Transporter GLUT3 also known as GLUT-3 or SLC2A3 serves as an important protein in glucose transport across cell membranes. It is part of the facilitative glucose transporter family and exhibits a molecular weight of approximately 54 kDa. This transporter shows high affinity for glucose making it particularly important in cells with high demands for glucose. You mainly find GLUT3 expressed in brain neurons placenta and sperm where it ensures sufficient glucose uptake to sustain energy-intensive activities.
Biological function summary
The transporter protein mediates the rapid uptake of glucose into cells especially under low glucose concentrations supporting high cellular metabolic rates. It does not function alone but collaborates in transporting glucose to meet cellular needs efficiently. Although GLUT3 operates as a specific transporter it is involved in a larger network that ensures glucose availability for vital cellular processes ensuring that cells like neurons maintain function even under varying glucose availability.
Pathways
The transporter plays an integral role in the glycolysis and cellular respiration pathways. It acts in concert with other glucose transporters such as GLUT1 and GLUT4 which also facilitate glucose uptake into various cells allowing efficient energy production. The proper function of glycolysis heavily depends on the effective operation of glucose transporters where GLUT3's high affinity for glucose supports metabolic activities specifically in neural cells which relies on glucose as a primary energy source.
Several studies link GLUT3 to conditions like Alzheimer's disease and Type 2 diabetes. In Alzheimer's impaired glucose metabolism in the brain partly due to dysfunctional GLUT3 contributes to neurodegenerative processes. Similarly altered expression of GLUT3 affects glucose homeostasis playing a part in Type 2 diabetes pathophysiology alongside proteins like insulin receptors and other glucose transporters. These associations underline the significance of maintaining GLUT3 function for cellular glucose management and metabolic health.