Abcam, ab301281, Human TRAP1 knockout A549 cell line

Size: 1000000Cells / vial
TRAP1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Disease:Carcinoma

Product details:
Recommended control
: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRAP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRAP1 also known as Tumor Necrosis Factor Receptor-Associated Protein 1 or Heat Shock Protein 75 (Hsp75) is a mitochondrial chaperone protein with a molecular weight of approximately 75 kDa. It functions to protect cells from stress-induced damage by ensuring proper protein folding and preventing aggregation within the mitochondria. TRAP1 is expressed across various tissues with higher levels observed in metabolically active organs such as the heart and liver. Its role extends to regulation of mitochondrial respiration and reactive oxygen species indicating its broad involvement in cellular homeostasis.
Biological function summary
This mitochondrial chaperone plays an important role in maintaining protein homeostasis within the cellular environment. It forms part of a complex with heat shock proteins coordinating cellular defense mechanisms against stress. TRAP1 interacts with other chaperones to stabilize proteins and assist in mitochondrial quality control. Its activities aid in reducing oxidative stress managing apoptosis and regulating mitochondrial dynamics. Through these functions TRAP1 supports cellular survival and metabolic adaptation under conditions of stress.
Pathways
TRAP1 is involved in the regulation of the mitochondrial apoptosis pathway and the oxidative stress response pathway. It interacts with proteins like cytochrome c and inhibitors of apoptosis proteins (IAPs) influencing the release of pro-apoptotic factors and modulation of survival signals. By modulating these pathways TRAP1 affects cellular energy production and stress responses impacting both cellular health and disease states.
TRAP1 shows a direct link to cancer and neurodegenerative diseases. Overexpression of TRAP1 has been associated with various cancers where it promotes cell survival and resistance to apoptosis. In cancer cells TRAP1 interacts with proteins like p53 and Hsp90 contributing to the oncogenic process. In neurodegenerative disorders dysfunction of TRAP1 is linked to increased oxidative stress and mitochondrial dysfunction often relating to proteins such as PINK1 and Parkin which are important in the pathology of Parkinson’s disease.