Abcam, ab302642, Anti-TetR antibody [31B3]

Size: 20µg / 100µg / 1mg
Mouse Recombinant Monoclonal TETR2 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Escherichia coli, Transfected cell line - Escherichia coli samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:31B3,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Escherichia coli,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TetR or tetracycline repressor protein is a regulatory protein that controls the expression of genes involved in resistance to tetracycline. It weighs about 23 kDa. TetR functions as a homodimer binding to substrate DNA sequences to suppress transcription. This repression occurs by binding to the operator sequences of the tetracycline-inducible promoter blocking the progression of RNA polymerase along the DNA strand. TetR mainly operates within the bacterial species Escherichia coli serving as a well-characterized model for gene regulation in prokaryotes.
Biological function summary
TetR plays a critical role in antibiotic resistance mechanisms. When tetracycline is not present TetR maintains its binding to the operator effectively silencing the expression of downstream genes responsible for drug efflux. Upon binding tetracycline the protein undergoes a conformational change that releases the operator sequence allowing the transcription of resistance genes. TetR forms a complex through dimerization which is essential for its function as it binds the operator in pairs.
Pathways
TetR is integral to the regulation of the tetracycline resistance pathway. It is part of a classic feedback loop that includes TetA a protein involved in tetracycline efflux out of the cell. The TetR-TetA regulatory system finely tunes bacterial response to the presence or absence of tetracycline ensuring survival in antibiotic environments. Its interaction with the TetA protein highlights its essential role in regulating efflux pump expression during antibiotic exposure.
TetR connects primarily to antibiotic resistance a major issue in bacterial infections. The misuse of tetracycline in clinical settings emphasizes the significance of TetR in developing tetracycline-resistant bacterial strains. This protein's connection to the TetA efflux pump directly affects the efficacy of tetracycline as an antibacterial treatment. Understanding the TetR regulation mechanism could lead to new therapeutic strategies in combating drug-resistant pathogens particularly methicillin-resistant Staphylococcus aureus (MRSA).