Abcam, ab303504, Anti-ABCA4 antibody [EPR26543-66]

Size: 10µL-TRIAL / 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ABCA4 antibody. Suitable for WB, IHC-P, ICC/IF, mIHC and reacts with Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26543-66,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:WB, IHC-P, mIHC, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ABCA4 protein also known as ATP-binding cassette sub-family A member 4 is a large transporter protein with a molecular mass of around 250 kDa. It is located primarily in the retina specifically in the photoreceptor cells where it plays a critical role in the visual cycle. ABCA4 helps transport N-retinylidene-phosphatidylethanolamine and other retinoid compounds across photoreceptor disc membranes. This function is essential in maintaining the photoreceptors' integrity and function.
Biological function summary
ABCA4 has an important function in the clearance of retinal pigments and it operates as part of a larger complex with other visual cycle proteins. Its activity prevents the accumulation of toxic substances that can damage photoreceptors. ABCA4's transport activity is ATP-dependent meaning it requires energy from ATP hydrolysis to perform its duties. This energy consumption highlights the protein's dynamic role in retinal health and visual processes.
Pathways
ABCA4 is an important component in the visual cycle and the retinoid cycle pathway in rods and cones. These pathways are vital for regenerating visual pigments and ensuring proper vision. ABCA4 works alongside related proteins like RPE65 and RDH12 both of which also contribute to retinoid processing and photoreceptor function. The proper flow of retinoids and prevention of toxic buildup depend heavily on ABCA4 and these associated proteins.
ABCA4 is closely linked with Stargardt disease and age-related macular degeneration. Mutations in the ABCA4 gene disrupt its normal function leading to the accumulation of toxic retinoid byproducts that damage photoreceptor cells and impair vision. Such mutations connect ABCA4 with proteins like CRALBP and LRAT whose dysfunctions similarly affect the visual cycle contributing to the progression of retinal diseases.