Abcam, ab304939, Human TrkA ELISA Kit

Size: 1 x 96Tests
Human TrkA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TrkA with a sensitivity of 9.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 9.4 pg/mL,
Range:11.719 - 750 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human TrkA SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TrkA protein in human cell extract. Quantitate Human TrkA with 9.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TrkA also known as NTRK1 is a neurotrophin receptor and a member of the tropomyosin receptor kinase (TRK) family. It has a molecular mass of approximately 140 kDa. TrkA is expressed mostly in neuronal tissues particularly in the sensory ganglia sympathetic neurons and certain portions of the central nervous system. Mechanically TrkA activates upon binding to nerve growth factor (NGF) leading to receptor dimerization and autophosphorylation triggering downstream signaling cascades essential for neuronal growth and survival.
Biological function summary
TrkA plays an important role in the regulation of neurotrophic signaling. TrkA existing often as part of larger signaling complexes modulates neuronal differentiation development and apoptosis. It ensures proper neural connections during development by responding to NGF which is important for neurons' health and plasticity. TrkA also aids in the response to injury by promoting neuronal repair and regeneration.
Pathways
TrkA engagement activates prominent pathways like the MAPK/ERK and PI3K/AKT signaling cascades. These pathways facilitate important cellular processes like proliferation survival and metabolism. Through these pathways TrkA interacts and cooperates with proteins like SHC GRB2 and PLCγ among others to execute cellular functions. These signaling pathways enable the coordinated response needed for neural tissue maintenance and development.
TrkA abnormalities are linked to conditions like congenital insensitivity to pain with anhidrosis (CIPA) and certain cancers. Mutations in NTRK1 can disrupt NGF signaling leading to neurological deficits observed in CIPA. In cancer aberrant TrkA signaling often due to gene fusions contributes to tumorigenesis. TrkA-related signals influence oncogenic proteins such as RAS and BRAF illustrating its role in cancer progression.