Abcam, ab305073, Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal INSM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23199-37-6-1,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization.Human and rat are recommended based on IHC results. we do not guarantee western blot for human and rat.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The INSM1 protein also known as Insulinoma-Associated 1 is a zinc finger transcription factor with a molecular weight of about 60 kDa. This protein is expressed in developing and mature neuroendocrine tissues. It plays an important role in the development of the nervous system and serves as a marker for neuroendocrine differentiation. Researchers can use INSM1 immunohistochemistry (IHC) to identify neuroendocrine tumors.
Biological function summary
The transcription factor INSM1 regulates key genetic programs necessary for neuronal differentiation. It interacts with other transcription factors and is involved in the repression of specific neuronal genes. During neural development it promotes the maturation of progenitor cells into specialized neuroendocrine cells. INSM1 acts as part of a transcriptional complex that modulates important developmental genes in the central nervous system.
Pathways
INSM1 plays a critical role in the Notch signaling and Hedgehog pathways. In the Notch signaling pathway it contributes to neuronal differentiation by regulating the expression of Hes1 and other Notch target genes. The Hedgehog pathway involvement suggests a role in mediating signals necessary for cellular patterning during neurogenesis. INSM1 interacts with proteins like Hes1 and Gli1 driving the cascade of events that lead to neuronal development and differentiation.
INSM1 relates to neuroendocrine tumors and diabetes. In neuroendocrine tumors INSM1 expression assists in tumor identification and is a diagnostic marker due to its specificity for these tissues. Additionally disruptions in INSM1 function can impact pancreatic beta-cell development highlighting its relevance to diabetes. Its relation to proteins such as PDX-1 and Nkx6.1 is important in understanding its involvement in disease mechanisms particularly in neuroendocrine and metabolic disorders.