Abcam, ab305325, Anti-Smad2 + Smad3 antibody [18/Smad2/3]

Size: 20µg / 100µg / 1mg
Mouse Recombinant Monoclonal SMAD2 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:18-Smad2-3,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat, Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Smad2 and Smad3 also known as Smad family member 2 and Smad family member 3 are intracellular proteins involved in signaling cascades. Smad2 has a molecular mass of approximately 58 kDa and Smad3 is around 52 kDa. These proteins are part of the Smad family which functions in the signal transduction for the Transforming Growth Factor-beta (TGF-β) superfamily. Smad2 and Smad3 are widely expressed in many tissues including the brain heart and lung suggesting their broad biological roles.
Biological function summary
Smad2 and Smad3 regulate transcriptional responses essential for cellular processes including proliferation differentiation and apoptosis. These proteins form a trimeric complex with Smad4 upon activation by TGF-β receptors. The activated complex translocates into the nucleus where it binds to DNA and influences gene expression. Such actions highlight their fundamental role in controlling cell behavior and fate.
Pathways
Smad2 and Smad3 play critical roles within the TGF-β signaling pathway. This pathway has significant effects on cell cycle control and immune regulation. Both Smad2 and Smad3 interact closely with the protein Smad4 to mediate various downstream effects integrating signals with other pathways such as MAPK pathway which involves different cellular responses.
Smad2 and Smad3 are closely associated with cancer and fibrosis. Aberrant TGF-β signaling often leads to tumor progression and metastasis partly due to the dysregulated actions of Smad2 and Smad3. Additionally these proteins through their pathway functions contribute to the pathological process of fibrosis in organs like the liver and lungs. It is important to note their interaction with the ERK protein which can also influence disease outcomes in these contexts.