Abcam, ab305337, Anti-KMT4/Dot1L antibody [EPR22936-138] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal KMT4 / Dot1L antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22936-138,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KMT4 also known as Dot1L is a histone methyltransferase that catalyzes the methylation of histone H3 at lysine 79. This protein plays a significant role in chromatin remodeling and gene expression regulation. Dot1L has a molecular mass of approximately 171 kDa. It expresses widely in various tissues with high presence in the heart brain and testes indicating its importance in diverse cellular processes.
Biological function summary
Dot1L functions as a critical enzyme influencing transcriptional activation and repression. It does not require a specific formed complex to execute its activity unlike many other methyltransferases that work as part of larger protein complexes. Dot1L interacts directly with nucleosomes and influences gene expression by modifying chromatin structure. This regulation of gene expression is important for the coordination of normal developmental processes and cellular proliferation.
Pathways
Dot1L impacts epigenetic regulation and is integral to the Wnt signaling pathway. This pathway is essential for processes like embryonic development and cell differentiation. Dot1L also plays an essential role in DNA damage response and repair pathways. It collaborates with other proteins like BRG1 a chromatin re-modeling factor to initiate specific genomic responses related to transcriptional control genome stability and repair mechanisms.
Dot1L has been linked to mixed lineage leukemia (MLL) and other types of hematological malignancies. The aberrant activity of Dot1L can disrupt normal gene regulatory mechanisms leading to uncontrolled cell proliferation and cancer. Dot1L interacts with MLL fusion proteins that result from chromosomal translocations which are often present in leukemia. Abnormal Dot1L activity is also associated with the progression of other cancers making it a potential therapeutic target in oncology.