Abcam, ab307306, Anti-alpha + beta Synuclein antibody [EPR1646-65] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal beta Synuclein antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1646-65,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-synuclein and beta-synuclein are proteins implicated in the dynamics of neuronal function. Alpha-synuclein also known as SNCA is a synaptic protein weighing approximately 14 kDa expressed highly in the central nervous system. Unlike alpha-synuclein beta-synuclein shares a similar structure but differs in function and molecular weight. Both proteins belong to the synuclein family which also includes gamma-synuclein and are found primarily in pre-synaptic terminals contributing to synaptic vesicle physiology.
Biological function summary
Alpha- and beta-synuclein play roles in synaptic function and plasticity where they influence neurotransmitter release and vesicle recycling. These proteins do not generally form a stable complex with each other but their presence together suggests a regulatory interplay. Alpha-synuclein may promote vesicle formation and trafficking while beta-synuclein appears to counteract alpha's effects potentially protecting neurons from alpha-synuclein aggregation.
Pathways
Alpha- and beta-synuclein are key components in the synaptic vesicle cycle especially associated with the SNARE complex and neurotransmitter balance. Alpha-synuclein interacts with proteins like VAMP2 and STX1A in vesicle merging while beta-synuclein's regulatory role has implications in maintaining these interactions. In lipid metabolism alpha-synuclein influences fatty acid binding protein pathways impacting neuronal lipid transport and homeostasis.
Alpha- and beta-synuclein hold significant relevance particularly in neurodegenerative disorders like Parkinson's disease and dementia with Lewy bodies which show marked alpha-synuclein aggregates known as Lewy bodies. Alpha-synuclein's accumulation in neurons correlates with disease progression. Beta-synuclein on the other hand appears to have a protective role potentially mitigating the toxicity of alpha's aggregates. The relation between these synucleins underlines their importance in therapeutic research for these disorders.