Abcam, ab307450, Anti-Polyoma virus, Large T antigen antibody [CM2B4]

Size: 20µg / 100µg / 1mg
Mouse Recombinant Monoclonal Large T antigen antibody. Suitable for WB, IHC-P and reacts with Merkel cell polyomavirus samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CM2B4,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Merkel cell polyomavirus,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Polyoma virus Large T antigen also known as TAg is a protein that plays an important role in the viral life cycle. It has a molecular mass of approximately 100 kDa. TAg is expressed in infected cells during the early phase of virus replication. It binds to the viral origin of replication to initiate DNA synthesis and has helicase activity which unwinds the DNA. This protein also binds to various cellular proteins including p53 and pRb leading to disruption of regular cellular functions and promoting viral replication.
Biological function summary
Large T antigen acts as a multifunctional protein involved in both viral replication and cell transformation. It is critical for the assembly of replication complexes. TAg interacts with host proteins to promote cell proliferation and prevents apoptosis which is a programmed cell death. This interaction forms a complex that is important in driving the cell into S-phase where DNA replication occurs. Large T antigen mimics normal cellular signals to facilitate uncontrolled cell division.
Pathways
Large T antigen connects to significant regulatory mechanisms involved in cell cycle control. It acts in the RB tumor suppressor pathway by inactivating the retinoblastoma protein family including pRb. This disruption allows cells to bypass normal growth signals leading to continuous entry into the S-phase. Large T antigen also impacts the p53 pathway inhibiting this tumor suppressor protein and further promoting cell cycle progression and DNA replication similar to pathways seen in other oncogenes.
Large T antigen associates notably with various cancers and polyomavirus-related diseases. It contributes to oncogenesis by interfering with the tumor suppressor functions of p53 and pRb proteins both well-known for their roles in preventing cancer. Large T antigen's interactions with these proteins disrupt normal cell cycle regulation and promote the development of tumors such as in cases of Merkel cell carcinoma and JC virus-related progressive multifocal leukoencephalopathy. These interactions highlight the significant role of Large T antigen in disease manifestation particularly in immune-compromised individuals.