Abcam, ab307555, Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Swd2 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27034-63,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Mouse, Human,
Applications:Flow Cyt (Intra), ICC/IF, IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Swd2 also known as Cwc21 or Swd2p is a protein that functions as a regulatory component in RNA splicing and transcription. This protein has a mass of approximately 47 kDa and is primarily expressed in the nucleus of eukaryotic cells. Swd2 associates with the spliceosome and transcription-related complexes playing a role in coordinating transcription and processing of RNA. Understanding its precise mechanical role is key to appreciate its broader biological implications.
Biological function summary
Swd2 operates as part of the COMPASS complex a multiprotein assembly involved in histone modification. This complex methylates histone H3 at lysine 4 (H3K4) influencing chromatin structure and gene expression regulation. Swd2 contributes to the function of the spliceosome by interacting with core splicing machinery components aiding the removal of introns from pre-mRNA. Its activity affects gene expression patterns linking transcription processes with RNA maturation in the cell.
Pathways
Swd2 is integral within two major biological pathways: RNA splicing and chromatin modification. Through RNA splicing it assists with the precise excision of introns interacting with proteins like Prp19. In the chromatin modification pathway it acts as part of the COMPASS complex which involves Set1 in the methylation of H3K4. This methylation is critical for chromatin remodeling therefore impacting transcription regulation and gene silencing.
Researchers have noted associations between Swd2's function and certain cancers and neurodegenerative disorders. Aberrations in RNA splicing regulation where Swd2 is involved may contribute to oncogenesis. Altered Swd2 expression or function can disrupt the careful balance required for normal cell proliferation. Increased aberrant splicing or disrupted trimethylation at H3K4 potentially linked to Set1 may lead to pathways involved in these disorders. Understanding Swd2's pathways could be important for therapeutic exploration.