Abcam, ab307612, Alexa Fluor® 647 Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65]

Size: 100µL
Rabbit Recombinant Monoclonal Alpha-synuclein antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Transfected cell line samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25115-65,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-synuclein also known as alpha-synuclein kDa or isoform ANX-112 is a protein with a molecular mass of approximately 14 kDa. It is mostly expressed in the brain especially in the presynaptic terminals of neurons. Structurally it is a small cytosolic protein that can form amyloid fibrils and is sensitive to chemical modifications. Alpha-synuclein plays a role in the regulation of neurotransmitter release and neuronal plasticity though its precise mechanisms remain an area of active investigation.
Biological function summary
Alpha-synuclein interacts with various membrane phospholipids and can form insoluble aggregates. These aggregates are part of complex structures known as Lewy bodies prominent in neurodegenerative conditions. This protein is implicated in synaptic vesicle trafficking maintaining synaptic function and neuronal adaptive responses. Alpha-synuclein may also modulate enzyme activities and participate in genomic stability reflecting its versatile cellular roles.
Pathways
Alpha-synuclein is involved in the dopamine synthesis pathway and membrane trafficking pathways. In these pathways it interacts with proteins like synapsin and SNAREs influencing vesicle fusion and neurotransmitter packaging. Its association with such pathways highlights its role in maintaining neuronal integrity and communication. Dysregulation of alpha-synuclein's normal function might disrupt these pathways resulting in pathological consequences.
Alpha-synuclein is most commonly linked to Parkinson's disease and Lewy body dementia. In these conditions abnormal accumulation and aggregation of alpha-synuclein into Lewy bodies occur. These aggregates impede cellular function and promote neuronal death. Additionally its relationship with other proteins like tau in the neurodegenerative disease context highlights its significant impact in multiple disorders associated with cellular aging and synaptic dysfunction.