Abcam, ab307620, Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

Size: 100µL
Rabbit Recombinant Monoclonal MIP-1 alpha/CCL3 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22529-19,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MIP-1 alpha also known as CCL3 and CCL3L1 is a chemokine with a core protein mass around 8 kDa. It is primarily expressed in activated T cells monocytes and lymphocytes. CCL3 plays a role in chemotaxis by binding to receptors CCR1 and CCR5 on the surface of target cells. This interaction leads to cell signaling events that control the migration and activation of specific immune cells to sites of inflammation or infection.
Biological function summary
The molecule acts as an important mediator in the immune response by recruiting and activating neutrophils eosinophils and other leukocytes. It supports inflammatory responses particularly in allergic reactions and autoimmunity. While not a part of a traditional complex MIP-1 alpha directly interacts with its receptors to initiate immune signaling pathways. Its expression can be upregulated by pro-inflammatory cytokines thereby enhancing its biological effects during immune system activation.
Pathways
MIP-1 alpha is closely intertwined in the chemokine signaling pathway and plays an important role in the inflammatory response pathway. Within these pathways MIP-1 alpha coordinates with proteins like CCL5 (RANTES) and interleukin-8 facilitating the recruitment of immune cells. Its function provides a pivotal control point in the migration and activation of these cells influencing the overall immune response.
MIP-1 alpha is strongly associated with rheumatoid arthritis and human immunodeficiency virus (HIV) infection. The chemokine contributes to the inflammatory milieu present in rheumatoid arthritis by promoting the recruitment of inflammatory cells to the synovial membrane. In HIV MIP-1 alpha competes with the virus for binding to CCR5 receptors influencing disease progression. It shares its target receptor CCR5 with proteins such as CCL4 (MIP-1 beta) highlighting its importance in the context of viral diseases and autoimmune conditions.