Abcam, ab307837, Anti-NPAT antibody [27/NPAT]

Size: 10µg-TRIAL / 20µg / 100µg / 1mg
Mouse Recombinant Monoclonal NPAT antibody. Suitable for ICC/IF and reacts with Human samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:27/NPAT,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NPAT also known as nuclear protein ataxia-telangiectasia locus is a protein that weighs approximately 220 kDa. It is widely present in various tissues with a high expression in proliferating cells. NPAT participates in cell cycle regulation and links directly to the control of histone gene expression during the G1/S phase transition. Its presence in the nuclear matrix further highlights its integrative role in gene regulation.
Biological function summary
NPAT plays an important role in the cell cycle's advancement by associating with the MMB (Myb-MuvB) complex. This interaction signifies its importance in DNA replication and cell cycle progression. NPAT activates transcription upon phosphorylation by CDK2/cyclin E influencing S-phase entry. It acts as a transcriptional regulator for histone gene expression necessary for chromatin structure and genome stability.
Pathways
NPAT integrates into the cyclin/CDK regulatory pathway governing cell cycle progression and is related to histone gene clusters regulation. The interaction with CDK2 illustrates its part in the regulation of cell proliferation. NPAT also associates with the E2F cell cycle pathway further influencing gene transcription important for DNA replication.
NPAT relates to cancer and ataxia-telangiectasia. These connections highlight its importance in cell cycle control disruption which can lead to oncogenesis. NPAT's relationship with the ATM protein emerges in the context of ataxia-telangiectasia suggesting a link between DNA damage response defects and neurological disorders. These associations reinforce NPAT’s involvement in maintaining cellular health and genomic integrity.