Abcam, ab307998, Anti-PAX3 antibody [RP23040034]

Size: 100µg
Rabbit Recombinant Multiclonal PAX3 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PAX3.
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:RP23040034,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PAX3.P23760

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies

Properties and Storage Information:
Form-Liquid, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PAX3 also known as Paired Box 3 is a transcription factor that plays a critical role in embryonic development. It carries a molecular mass of approximately 53 kDa. PAX3 expression occurs mainly in the neural tube somites and limb buds where it regulates gene expression necessary for tissue and organ development. PAX3 protein consists of a paired domain and a homeodomain enabling it to bind DNA and control the expression of target genes. In Australia and abroad researchers often rely on specific PAX3 antibodies to study its presence and role in various biological contexts.
Biological function summary
This protein regulates cell proliferation migration and differentiation within the developing nervous system and skeletal muscles. PAX3 forms part of a complex network that influences various developmental pathways. It works in concert with other transcription factors to ensure proper formation of muscle and neural structures. By modulating these processes PAX3 ensures that tissues develop correctly and function in a coordinated manner.
Pathways
Researchers have identified PAX3’s involvement particularly in the development of neural crest cells and myogenesis. It participates in the Wnt signaling pathway where it interacts with proteins like beta-catenin to drive muscle precursor cell formation. Additionally PAX3 has a critical role in the Notch signaling pathway where it overlaps with the function of other transcription factors like MYOD in regulating muscle differentiation. These interactions highlight PAX3's significance in developmental processes.
Mutations in the PAX3 gene have strong associations with Waardenburg syndrome and alveolar rhabdomyosarcoma. In Waardenburg syndrome improper PAX3 function leads to defects in melanocyte differentiation impacting pigmentation and hearing. The connection between PAX3 and alveolar rhabdomyosarcoma involves fusion with the FOXO1 gene forming a chimeric protein that drives oncogenesis. Understanding PAX3’s interactions with other proteins such as MITF in melanocyte function opens avenues for potential therapeutic interventions.