Abcam, ab308407, Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free

Size: 100µL / 1mL
Mouse Monoclonal Dengue virus 2 Capsid protein antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Dengue virus 2 samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:GT574,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Dengue virus 2,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dengue Virus 2 often called Dengue Type 2 is a serotype of the dengue virus a significant vector-borne pathogen. The virus has a positive-sense single-stranded RNA genome encoding several structural and non-structural proteins including the capsid protein and NS5. The capsid protein weighing approximately 12 kDa is important for encapsulating the viral RNA forming the nucleocapsid. NS5 with a mass around 103 kDa functions as a methyltransferase and RNA-dependent RNA polymerase essential for the viral replication cycle. These proteins are expressed predominantly in host cells after the virus infects facilitating its life cycle and pathogenesis.
Biological function summary
Within host cells the capsid protein and NS5 are involved in viral assembly and RNA synthesis respectively. The capsid protein enables efficient packaging of the viral genome interacting with several cellular components for the assembly of new virions. NS5 engages in the methylation of viral RNA and replication of the viral genome. NS5 does not act alone; it works as part of a larger replication complex involving other viral proteins such as NS3 which provides helicase activity further supporting the functionality of the viral replication machinery.
Pathways
Dengue Virus 2 proteins play key roles in the viral replication and immune evasion pathways. The replication complex containing NS5 and other non-structural proteins orchestrates the replication pathway ensuring the production of viral progeny. The capsid protein is associated with the budding of new virions within the endoplasmic reticulum which is important for the release of infectious particles. In addition NS5 interacts with host proteins such as STAT2 modulating immune pathways to conceal the virus from host defenses enabling persistent viral replication and infection.
Dengue Virus 2 is related to Dengue fever and severe forms like Dengue hemorrhagic fever. The NS5 protein through its interaction with STAT2 plays a role in immune suppression affecting disease outcomes. The capsid protein's strong involvement in viral assembly links it to the efficient dissemination of the virus contributing to the severity and viral load in dengue infections. Understanding these interactions is essential for future therapeutic strategies aimed at mitigating dengue-related health issues worldwide.