Abcam, ab308632, Anti-GATAD2B antibody [EPR27329-3] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GATAD2B antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27329-3,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GATAD2B also known as p66β is a protein which forms part of the nucleosome remodeling and deacetylase (NuRD) complex. The protein has a mass of approximately 66 kDa. It is expressed in various human tissues including brain and skeletal muscle. GATAD2B plays a vital role in transcriptional repression by recruiting chromatin remodeling factors which influence gene expression regulation.
Biological function summary
GATAD2B influences chromatin structure and gene expression. It functions as an integral component of the NuRD complex which combines ATP-dependent chromatin remodeling with histone deacetylase activity. The interaction of GATAD2B with other NuRD components such as MBD2 and CHD3 demonstrates its role in modifying the accessibility of DNA to transcription factors impacting cellular activities like differentiation.
Pathways
Other components of the NuRD complex work together with GATAD2B in the regulation of the Wnt signaling pathway and the Notch signaling pathway. These pathways are important for cellular proliferation and differentiation. GATAD2B has functional connections with other proteins including REST and HDAC1 and these connections indicate its involvement in these pathways that shape developmental processes and cellular responses.
GATAD2B is significant in connection with neurodevelopmental disorders. Pathogenic mutations in the GATAD2B gene have been linked to GATAD2B-associated neurodevelopmental disorder. It is also associated with intellectual disability syndromes. GATAD2B shows interactions with proteins such as TCF4 and SMARCA2 which are also linked to similar neurodevelopmental conditions illustrating its impact on brain function and development.