Abcam, ab309109, Human Protein S ELISA Kit

Size: 1 x 96Tests
Human Protein S ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Protein S in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, EDTA Plasma, Citrate plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.216 ng/mL,
Range:0.469 - 30 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Protein S SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Protein S protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Protein S with 0.216 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein S also known as PROS1 is a vitamin K-dependent plasma glycoprotein with a molecular mass of approximately 75 kDa. It is synthesized mainly in the liver but also found in other tissues such as the endothelium megakaryocytes and osteoblasts. This protein plays a significant role in the regulation of blood coagulation by serving as a non-enzymatic cofactor for activated protein C (APC) in the proteolytic degradation of coagulation factors Va and VIIIa.
Biological function summary
The integrated function of Protein S within the coagulation cascade cannot be overemphasized. It acts synergistically with APC to limit thrombin generation therefore facilitating anticoagulation. Protein S is part of a complex with APC that exerts a potent anticoagulant effect. While free Protein S shows functional activity a large fraction circulates in complex with C4b-binding protein where it exhibits limited cofactor activity.
Pathways
The anticoagulant role of Protein S anchors it within the critical pathways like the protein C pathway impacting hemostasis. It directly associates with proteins such as Factor Va and Factor VIIIa and indirectly with proteins like thrombin. The regulation of coagulation through these pathways highlights its contribution to maintaining vascular integrity and preventing thrombosis.
Protein S deficiency has clinical implications in thrombotic conditions including deep vein thrombosis (DVT) and pulmonary embolism (PE). Such deficiencies can be hereditary or acquired often linked to disorders affecting vitamin K metabolism or liver function. Protein C another key anticoagulant closely interacts with Protein S in these disorders highlighting their collaborative effort in preventing excessive clot formation.