Abcam, ab309160, Anti-HIV1 p55 + p24 + p17 antibody [RM2002] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Multiclonal GAG antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB, I-ELISA and reacts with Transfected cell line - Human immunodeficiency virus, Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell lysate - Human immunodeficiency virus samples.
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:RM2002,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human immunodeficiency virus,
Applications:ICC/IF, Flow Cyt (Intra), IHC-P, I-ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIV-1 p24 HIV-1 p17 and HIV-1 p55 are essential proteins in the lifecycle of the Human Immunodeficiency Virus type 1 (HIV-1). HIV-1 p24 also known as the capsid protein weighs approximately 24 kDa and forms the conical core that houses viral genetic material. HIV-1 p17 or the matrix protein is around 17 kDa and contributes to the viral envelope's structure aiding in its formation and stabilization during the virus assembly. HIV-1 p55 known as the Gag polyprotein weighs around 55 kDa and is expressed in host cells infected by the virus. It undergoes proteolytic cleavage to produce p24 and p17 alongside other structural proteins which are critical for viral assembly and maturation.
Biological function summary
These proteins play instrumental roles in HIV-1's replication and assembly. HIV-1 p24 and p17 form part of the Gag protein complex which is necessary for particle formation and genome packaging. The p55 Gag precursor orchestrates virion assembly at the plasma membrane of the host cell. The Gag polyprotein drives the budding process where newly formed virions detach from the host cell becoming infectious particles. This compartmentalization of functions results in efficient regulation and production of new viral particles ensuring the perpetuation of infection.
Pathways
These proteins function within the viral replication and assembly pathway an important aspect for HIV-1's lifecycle. The Gag polyprotein is significantly involved in the budding and maturation of the virus coordinating with host cell factors and other viral proteins like HIV-1 reverse transcriptase. This interplay is essential for the replication cycle. Additionally interactions with the cellular ESCRT (endosomal sorting complexes required for transport) pathway facilitate the release of virions highlighting their role in efficient viral propagation.
These proteins are intimately connected to the pathogenesis of acquired immunodeficiency syndrome (AIDS). HIV-1 targets CD4+ T cells leading to immune system dysfunction. The p24 protein serves as a biomarker for infection detectable during seroconversion. The immune response to these proteins correlates with disease progression wherein levels of p24 can indicate viral load. Additionally the expression and processing of these proteins relate to resistance mechanisms in highly active antiretroviral therapy (HAART). The interplay of Gag with proteins like HIV-1 integrase is critical for understanding therapeutic targets since these interactions facilitate the integration of viral DNA into the host genome.