Abcam, ab309197, Anti-Otoferlin antibody [EPR28223-1]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal Otoferlin antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Transfected cell line - Mouse, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28223-1,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Mouse,
Applications:WB, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The otoferlin protein also known as OTOF plays an important mechanical role in the auditory system by serving as an important synaptic vesicle component for neurotransmitter release in inner hair cells of the cochlea. It has a molecular mass of approximately 230 kDa. Otoferlin is expressed primarily in the cochlea particularly in the sensory hair cells essential for hearing. Its functionality hinges on its capacity to serve as a calcium-sensor facilitating the rapid exocytosis of neurotransmitter-filled vesicles at the synaptic ribbon.
Biological function summary
Otoferlin contributes to sound perception by coordinating the timely release of synaptic vesicles that transmit auditory signals to the brain. It is part of a complex network within the auditory pathway interacting with other proteins important for synaptic function including synaptotagmin. Significant for its role in calcium-binding otoferlin participates in the fine-tuning of synaptic transmission ensuring efficient signaling required for precise auditory processing.
Pathways
Otoferlin is integral to the auditory signaling pathways especially the cochlear nerve pathway responsible for conveying sound information to the brain. It actively participates in vesicular trafficking and synaptic vesicle cycle ensuring high fidelity auditory signal transduction. Otoferlin works closely with proteins such as synaptophysin and syntaxin which are involved in the vesicle fusion process highlighting its role in facilitating efficient neurotransmitter release.
Otoferlin has a significant association with auditory processing conditions. Mutations in the otoferlin gene lead to severe-to-profound hereditary hearing loss specifically a form of non-syndromic autosomal recessive hearing impairment (DFNB9). Through this condition otoferlin has connections to proteins involved in synaptic vesicle recycling malfunctions contributing to auditory neuropathy. Additionally otoferlin-related dysfunction implicates it in conditions affecting synaptic transmission stability which are vital considerations in therapeutic strategies for hearing loss.