Abcam, ab309278, Human PKC epsilon ELISA Kit

Size: 1 x 96Tests
Human PKC epsilon ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PKC epsilon with a sensitivity of 6.657 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 6.657 pg/mL,
Range:18.75 - 1200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human PKC epsilon SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PKC epsilon protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human PKC epsilon with 6.657 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein kinase C epsilon (PKC epsilon) also known as PKCε or epsilon protein is an important serine/threonine kinase involved in various cellular processes. It has a molecular weight of about 87 kDa. PKC epsilon is widely expressed in different tissues prominently in the brain heart skeletal muscle and endocrine tissues. It belongs to the novel PKC subfamily that responds to diacylglycerol and phospholipids without requiring calcium for activation.
Biological function summary
PKC epsilon plays roles in regulating cell survival migration and apoptosis. It is involved in forming signalling complexes with proteins such as receptor for activated C kinase (RACK) and frequently associates with scaffolding proteins. PKC epsilon contributes to the modulation of cytoskeletal reorganization exocytosis and neurotransmitter release impacting cellular communication and function significantly.
Pathways
PKC epsilon is integrated into significant signalling cascades including the MAPK/ERK and PI3K/Akt pathways. These pathways influence cell proliferation differentiation and survival. PKC epsilon interacts with proteins like Raf-1 and Akt further modulating signals for cellular responses. Its activity and interactions within these pathways highlight its importance in maintaining cellular homeostasis.
PKC epsilon has connections to cancer and cardiovascular diseases. It plays a part in oncogenic processes such as tumorigenesis and metastasis often working alongside proteins like PI3K in these contexts. In cardiovascular conditions PKC epsilon influences cardiac hypertrophy and ischemic heart damage. Its role associating with proteins such as endothelial nitric oxide synthase (eNOS) illustrates its involvement in disease mechanisms where blood flow and myocardial function are affected.