Abcam, ab309353, Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Protein X antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB, I-ELISA and reacts with Transfected cell lysate - Hepatitis B virus, Transfected cell line - Hepatitis B virus, Recombinant fragment - Hepatitis B virus samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27041-49,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Hepatitis B virus,
Applications:ICC/IF, I-ELISA, Flow Cyt (Intra), IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein X also known as HBV protein X is a multifunctional protein with a molecular mass of approximately 17 kDa. It is an integral component of Hepatitis B virus (HBV) and expressed within infected host liver cells. Protein X influences viral replication by interacting with host cellular mechanisms specifically modulating transcriptional regulation and signaling pathways that can benefit viral persistence. Due to its versatile nature researchers study Protein X's diverse roles in the context of HBV infection.
Biological function summary
The function of Protein X extends beyond viral replication. It plays a significant role in manipulating the host's immune response often suppressing antiviral activities which can allow the virus to evade the immune system. The interactions with cellular proteins may not always be direct as Protein X can be part of a larger viral replication complex. These interactions impact the host cell’s regulatory networks complicating the response to infection and facilitating chronic hepatitis conditions.
Pathways
Protein X is involved in the regulation of cellular apoptosis and the modulation of the NF-kB signaling pathway. These pathways contribute to the survival and proliferation of infected cells and Protein X often interacts with host proteins like p53 and CREB to exert its effects. By affecting these pathways Protein X ensures the survival of the virus within the host promoting pathological conditions.
Protein X's impact is most closely associated with chronic Hepatitis B and related liver diseases. The protein's involvement in hepatocarcinogenesis highlights its oncogenic potential since it can promote the development of hepatocellular carcinoma (HCC). Interactions with proteins such as p53 highlight its role in oncogenic processes disrupting cell cycle regulation and apoptosis which can lead to uncontrolled cell growth and cancer progression.