Abcam, ab310318, Recombinant HIV1 Gag protein (His-tag)

Size: 10µg / 50µg / 100µg
Recombinant HIV1 Gag protein (His-tag) is a HIV-1 M:B_MN Fragment protein, in the 136 to 366 aa range, expressed in Escherichia coli, with >95%, <0.1 EU/mg endotoxin level, suitable for Mass Spec, SDS-PAGE, HPLC.
Key facts
Purity:>95% HPLC,
Endotoxin level:<0.1 EU/mg,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, HPLC, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P05888,
Animal free:Yes,
Carrier free:No,
Species:HIV-1 M:B_MN,
Reconstitution:Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.,
Storage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Gag polyprotein also known as the Gag protein or HIV Gag is an essential component of the HIV virus. It is expressed predominantly in host cells infected with HIV-1 and HIV-2. The Gag protein organizes into a structural protein that facilitates viral assembly and release. It typically has a mass of around 55 kDa. Gag proteins localize primarily in the cytoplasm and assemble at the plasma membrane where they form virus-like particles (VLPs).
Biological function summary
The Gag polyprotein serves an essential role in the life cycle of HIV. It forms a major structural component of the virus particle and contributes to the virion's architecture. The Gag protein mediates the packaging of the viral RNA genome into new virus particles and is important for forming the matrix capsid and nucleocapsid of the virus. It is not typically considered part of a larger complex but forms its own multimeric assembly during virus budding.
Pathways
The Gag polyprotein is integral to the viral replication pathway and the process of viral budding from host cells. It interacts with host cell membranes and orchestrates the encapsulation of viral components into new virions. Critical pathways like the proteolytic maturation of viral proteins and assembly incorporate Gag proteins involving other viral proteins such as the Pol protein which helps in the processing and maturation of the viral structure.
The Gag protein is directly related to HIV/AIDS. The presence and functionality of the Gag protein enable the virus to replicate within the host contributing to the progression of the disease. The interaction between the Gag protein and other viral components like the Env protein is also essential for the viral entry into new cells which exacerbates the infection process. Efforts to inhibit the Gag polyprotein function are important in controlling HIV proliferation and managing HIV-related disorders.