Abcam, ab310799, Recombinant Human IL-18 Protein (His tag) (Active)

Size: 10µg / 50µg / 100µg
Recombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with >95%, <0.1 EU/mg endotoxin level, suitable for Mass Spec, SDS-PAGE, HPLC, Cell Culture.
Key facts
Purity:>95% HPLC,
Endotoxin level:<0.1 EU/mg,
Expression system:Escherichia coli,
Tags:His tag C-Terminus,
Applications:HPLC, Cell Culture, SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 107 units/mg.,
Accession:Q14116,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.,
Storage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient