Abcam, ab312811, iFluor® Red 750 Conjugation Kit

Size: 100µg
iFluor® Red 750 Conjugation Kit (ab312811) offers several standout features: - Rapid Conjugation: achieve iFluor® Red 750 labeling in under 2 hours with minimal hands-on time. - Labels up to 100 µg antibody. - Versatility: suitable for conjugating antibodies and proteins. iFluor® Red 750-labeled antibodies can be used immediately in applications such as Flow cytometry, ICC/IF and other fluorescence imaging assays without further purification.
Product details:
iFluor® Red 750 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Red Laser-excitable iFluor® Red 750. This dye is optimized to have minimal fluorescence quenching effect on proteins and nucleic acids, with a fluorescence maxima (Ex/Em) of 753/779 nm respectively. Comparison studies indicate that iFluor® 750 is significantly brighter than the corresponding Cy7® and Alexa Fluor® 750, These characteristics make it an excellent replacement for these dyes. iFluor® Red 750 is a stable dye and shows good reactivity and selectivity with protein amino groups.
This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of iFluor® Red 750 provided in the kit is optimized for labelling ~50 μg antibody
Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C