Abcam, ab313837, Anti-Artemis antibody [HL1227] - BSA and Azide free

Size: 100µL
Rabbit Recombinant Monoclonal Artemis antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCLRE1C.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1227,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human DCLRE1C.Q96SD1

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Artemis also known as DNA cross-link repair 1C (DCLRE1C) is a protein that plays an important role in DNA repair. It has a molecular mass of approximately 78 kDa. Artemis expresses mainly in cells of the immune system including lymphocytes and has been shown to contribute to maintaining genome stability. The protein acts as a nuclease which means it can cleave phosphodiester bonds within a nucleic acid chain allowing for the resolution of DNA double-strand breaks (DSBs).
Biological function summary
Artemis functions in the resolution of DNA double-strand breaks during processes such as V(D)J recombination and class switch recombination which are essential for the immune system's adaptive response. It forms a complex with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) which is essential for its activation. The complex is necessary for the non-homologous end joining (NHEJ) pathway a major pathway to repair DSBs. The proper functioning of Artemis ensures the integrity and diversity of the antigen receptor repertoire.
Pathways
Artemis plays a critical role in the non-homologous end joining pathway. This pathway is one of the two main mechanisms for repairing double-strand breaks in DNA the other being homologous recombination. Artemis works in conjunction with other proteins such as Ku70/80 and DNA ligase IV in NHEJ. Additionally Artemis's role in V(D)J recombination links it with key immune processes affecting the diversity of antibodies and T-cell receptors important for effective immune responses.
Improper function or mutations in the Artemis protein can lead to severe combined immunodeficiency (SCID) a disorder characterized by a lack of functional immune response. Artemis mutations relate to increased sensitivity to ionizing radiation linking it with radiosensitivity disorders. It also interacts with the protein ATM (ataxia-telangiectasia mutated) which is similarly involved in DNA damage response highlighting a connection between Artemis dysfunction and increased susceptibility to genomic instability-related disorders.