Abcam, ab313842, Anti-pan PKC (phospho T410) antibody [HL1279] - BSA and Azide free

Size: 100µL
Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Immunogen corresponding to Synthetic Peptide within Human PRKCZ phospho T410 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1279,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat, Drosophila melanogaster,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRKCZ phospho T410 conjugated to Keyhole Limpet Haemocyanin.Q05513

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein kinase C (PKC) family comprising isoforms like PKC alpha delta epsilon beta I gamma zeta eta theta and iota plays a significant role in cellular signaling. These isoforms also known as PRKCA PRKCD PRKCE PRKCB PRKCG PRKCZ PRKCH PRKCQ and PRKCI are serine/threonine kinases with masses ranging from 76 to 115 kDa. PKCs are expressed in various tissues including the brain heart muscle and immune cells. Mechanically they become activated through the phosphorylation driven by lipid second messengers such as diacylglycerol (DAG) and calcium ions which lead to their translocation to cellular membranes where they exert their effects.
Biological function summary
PKC isoforms are critical for regulating numerous cellular processes such as proliferation differentiation apoptosis and migration. They do not usually form complexes but interact with other kinases and phosphatases to transmit signals. They modulate essential cellular pathways ensuring various physiological responses are executed efficiently. Specific isoforms like PKC gamma show high expression in neuronal tissues influencing neuronal functions and synaptic plasticity.
Pathways
PKC isoforms are central to the MAPK/ERK and NF-kB signaling pathways. These pathways regulate important cellular responses including gene expression and stress responses. PKC works in conjunction with other kinases like RAF and IKK which are integral to these pathways providing structural and functional support. The activation of PKC can induce MAPK/ERK signaling which triggers cell proliferation and differentiation while its interaction with the NF-kB pathway can modulate immune responses and cell survival.
PKC isoforms have significant roles in cancer and cardiovascular diseases. Aberrant PKC activity affects cell cycle regulation and apoptosis contributing to tumor development and progression. PKC theta is linked to T-cell activation and autoimmunity contributing to autoimmune diseases. Additionally the dysregulation of PKC alpha and beta I is implicated in myocardial disorders by influencing cardiac contractility and hypertrophy often through interactions with calmodulin kinases and other cardiac-specific proteins. Understanding these interactions can aid in developing targeted therapies for such conditions.