Abcam, ab313844, Anti-NEK7 antibody [HL1348] - BSA and Azide free

Size: 100µL
Rabbit Recombinant Monoclonal NEK7 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEK7 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1348,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NEK7 conjugated to Keyhole Limpet Haemocyanin.Q8TDX7

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.
Biological function summary
Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.
Pathways
NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.
NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.