Abcam, ab313846, Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] - BSA and Azide free

Size: 100µL
Rabbit Recombinant Monoclonal SARS-CoV-2 nsp5 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell line - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1367,
Isotype:IgG,
Carrier free:Yes,
Reacts with:SARS-CoV-2,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 3CLpro also known as 3C-like protease or nsp5 is a protease enzyme with a critical role in the viral life cycle. It has an estimated mass of about 33.8 kDa. It performs its function primarily in the cytoplasm of the host cell. This protease cleaves the viral polyprotein at specific sites to generate functional proteins necessary for the viral replication and transcription process. It consists of a catalytic dyad made up of cysteine and histidine which aids in the hydrolysis of peptide bonds.
Biological function summary
The 3CLpro ensures the maturation of non-structural proteins necessary for SARS-CoV-2 replication. It is part of a larger polyprotein complex that the virus produces upon infection. The 3CLpro processes this polyprotein to release various functional viral proteins that aid in RNA synthesis and modification. It is a target for antiviral drug development due to its importance in viral propagation and lack of homologous human proteases reducing the likelihood of off-target effects.
Pathways
The function of 3CLpro is integral in the viral replication pathway of SARS-CoV-2. Within this pathway it interacts with viral RNA-dependent RNA polymerase essential for transcribing and replicating the viral genome. Another significant connection occurs with non-structural protein 8 (nsp8) where 3CLpro aids by cleaving the polyprotein to release the mature form of this accessory protein facilitating RNA replication.
SARS-CoV-2 3CLpro's function targets COVID-19 caused by the novel coronavirus. Its proteolytic activity is directly linked to the pathogenesis of COVID-19 as it enables viral replication within host cells. In this context 3CLpro is associated with other viral proteins such as nsp12 the main RNA polymerase critical for the replication process thereby representing a potential therapeutic target to disrupt viral life cycle and mitigate disease progression.