Abcam, ab313981, Human LAYN ELISA Kit

Size: 1 x 96Tests
Human LAYN ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human LAYN in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Serum, Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.012 ng/mL,
Range:0.012 - 10 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human LAYN ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LAYN in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human LAYN coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III (Tissue Factor/Thromboplastin), CTLA-4 (CD152), Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL36 alpha (IL1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3, TWEAK (TNFSF12)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LAYN protein also known as layilin plays a role in cell adhesion and signal transduction. It is an approximately 55 kDa protein expressed mainly in epithelial and immune cells including T-cells and natural killer cells. This protein sits on the cell surface and has a connection to the cytoskeleton through its cytoplasmic tail. Alternate expression may occur in both normal and cancerous tissues indicating a role in various physiological and pathological processes.
Biological function summary
LAYN facilitates interactions between cells and the extracellular matrix. It acts as a receptor for hyaluronic acid contributing to cell migration and tissue remodeling. While it is not part of any specific complex layilin may interact with other extracellular matrix proteins to promote cellular activities. This interaction influences immune responses by modulating immune cell activation and homing.
Pathways
LAYN is involved in the hyaluronan-mediated motility pathway where it binds hyaluronic acid to regulate cell movement. Additionally the protein takes part in the immune response pathway by influencing immune cell behavior. LAYN interacts with proteins like moesin which helps connect the cell membrane to the actin cytoskeleton driving cellular changes during these processes.
LAYN has a connection to cancer progression and autoimmune diseases. It plays a role in the tumor microenvironment affecting tumor cell motility and can influence metastasis. In autoimmune disorders LAYN's role in modulating immune cell activity can trigger aberrant immune responses. The protein interacts with various components of the immune system linking its expression to both health and disease states.