Abcam, ab313997, Anti-Giardia lamblia antibody [4G5]

Size: 1mg
Mouse Monoclonal Giardia lamblia antibody. Suitable for ICC/IF and reacts with Giardia lamblia samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4G5,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Giardia lamblia,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 Preservative: 0.097% Sodium azide Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Giardia lamblia often called Giardia is a flagellated protozoan parasite and its mechanical function is important in understanding giardiasis the disease it causes. Its trophozoite form which is around 10-20 µm long attaches to the intestinal epithelium of the host leading to infection. The organism uses its adhesive disc to interact with the host. The parasite is able to express various surface proteins to adapt to the host’s immune system; among these variant-specific surface proteins (VSPs) play a significant role in its survival and virulence.
Biological function summary
Giardia lamblia causes diarrhea and malnutrition due to malabsorption in the intestines. The organism disrupts the normal epithelial barrier which results in impaired digestion and nutrient uptake. It is not part of a complex but does produce cysts that resist harsh environmental conditions outside the host. These cysts facilitate transmission and protect the parasite until it reaches a new host. The detected antigens in the stool play an important role in diagnosis such as using tests like antigardia and giardia ELISA.
Pathways
The interaction of Giardia lamblia with the intestinal cells involves several significant biological processes. It disrupts host signaling pathways associated with tight junctions such as the actin cytoskeleton pathway. Related proteins like myosin light chain kinase (MLCK) become activated leading to alteration of epithelial barrier functions. This affects the integrity of intestinal tight junctions causing permeability issues and contributing to diarrhea.
Giardia lamblia is closely connected with giardiasis characterized by symptoms like diarrhea weight loss and abdominal discomfort. The disruption of the intestinal epithelial barrier by Giardia relates to another protein zonulin which is associated with increased intestinal permeability. Although usually self-limiting giardiasis can have significant impacts especially in children and immunocompromised individuals triggering other gastrointestinal disorders if not properly addressed.