Abcam, ab314175, Anti-14-3-3 beta antibody [6F6]

Size: 100µL
Rabbit Monoclonal 1433B phospho S184 + S186 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human YWHAB phospho S184 + S186.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:6F6,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human YWHAB phospho S184 + S186.P31946

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
14-3-3 beta also known as YWHAB is a protein that electrically neutralizes and stabilizes partner proteins. It has a mass of about 30 kDa and is part of the highly conserved 14-3-3 protein family. This family is ubiquitous in eukaryotic cells with 14-3-3 beta being expressed in many tissues including the brain and muscles. Its mechanical role involves binding phosphorylated serine and threonine residues which influences the conformation and function of the target protein.
Biological function summary
14-3-3 beta plays a central role in cellular processes like signal transduction apoptosis and cell cycle control. It often acts in concert with other proteins forming complexes that regulate these vital functions. For instance it binds to Raf-1 kinase affecting cell growth and differentiation. It also interacts with proteins involved in the mitogen-activated protein kinase (MAPK) pathway highlighting its diverse biological significance.
Pathways
14-3-3 beta is important in both the MAPK and apoptotic pathways. In the MAPK pathway it helps regulate the phosphorylation status of proteins involved in cell proliferation. The protein binds to Raf-1 an important component in MAPK signaling impacting cellular responses to growth signals. In the context of apoptosis 14-3-3 beta partners with BAD protein a pro-apoptotic factor sequestering it away from mitochondria this interaction is critical for cell survival by preventing the apoptotic process.
Disruptions in 14-3-3 beta function associate with neurodegenerative diseases and cancer. For example its interaction with proteins like Tau connected to Alzheimer's disease points to its involvement in neurodegeneration. Aberrations in its regulatory roles can also contribute to uncontrolled cell growth linked to cancer. Furthermore its interaction with Bcl-2 family proteins ties it to variations in apoptotic regulation seen in cancerous cells.