Abcam, ab314180, Anti-E2F4 antibody [24E12]

Size: 100µL
Rabbit Monoclonal E2F4 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human E2F4.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:24E12,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human E2F4.Q16254

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
E2F4 sometimes known as E2F-4 is part of the E2F family of transcription factors. It plays a critical role in controlling the cell cycle by regulating the expression of genes necessary for DNA synthesis. E2F4 has a molecular weight of around 45 kDa. These transcription factors are widely expressed in a variety of tissues reflecting their importance in cell biology. E2F4 typically functions by forming complexes with dimerization partner (DP) proteins mainly in the nucleus where it binds to DNA and influences transcription.
Biological function summary
E2F4 functions as a regulator of cell cycle progression. It forms complexes localizing mostly in the nucleus controlling the transition from G1 to S phase by regulating genes associated with cell cycle arrest and DNA replication. Partnering with members of the DP family enhances its DNA binding capacity influencing genes involved in cell cycle arrest and DNA repair processes. E2F4 predominantly acts as a transcriptional repressor in quiescent and differentiating cells maintaining the cell in a non-proliferative state.
Pathways
E2F4 is significant in the regulation of the cell cycle and DNA damage response pathways. It engages with the retinoblastoma protein family (pRB p107 p130) to exert repressive effects on E2F-target genes ensuring proper cell cycle exit and maintaining the G0 phase. The interaction with p130 is instrumental during the G0 state preventing inappropriate cell proliferation and contributing to the maintenance of cellular quiescence.
E2F4's role is noteworthy in cancer and retinoblastoma. Alterations in its regulation or activity can lead to unchecked cell proliferation a hallmark of cancerous growths. The malfunction or suppression of its activity can disrupt cell cycle control contributing to oncogenesis. In instances of retinoblastoma improper interaction between E2F4 and the retinoblastoma protein family can result in disrupted cell cycle checkpoints highlighting its importance in maintaining cellular homeostasis and preventing tumorigenesis.