Abcam, ab314428, Anti-PYGL (phospho S430) + PYGM (phospho S430) antibody [EPR20852-26]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal PYGL phospho S430 antibody. Suitable for WB, IP, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20852-26,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IP, WB, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PYGL (phospho S430) and PYGM (phospho S430) are phosphorylated forms of the enzymes glycogen phosphorylase liver (PYGL) and muscle (PYGM). These enzymes play fundamental roles in glycogen metabolism catalyzing the breakdown of glycogen into glucose-1-phosphate which can then be converted to glucose-6-phosphate for energy production. The phosphorylated form at the serine 430 residue is significant for regulation. PYGL is predominantly expressed in liver tissues while PYGM is found mainly in skeletal muscle. The mass of PYGL is approximately 97 kDa and PYGM is around 97 kDa as well.
Biological function summary
Glycogen phosphorylases participate in the glycogenolysis process providing energy during fasting or muscular activity. The enzymes operate as dimers or tetramers forming more active configurations when phosphorylated. PYGL helps maintain blood sugar levels during fasting by releasing glucose from liver glycogen stores. PYGM facilitates glucose availability during muscle contraction. Both forms influence energy balance and metabolic responses.
Pathways
Both PYGL and PYGM are central to carbohydrate metabolism and energy homeostasis. They are key components of the glycogen breakdown pathway which intersects with glycolysis. These enzymes interact with proteins like AMP-activated protein kinase (AMPK) which influences their activity through phosphorylation status. This regulation connects to pathways like the insulin signaling pathway controlling glucose production and uptake.
Glycogen phosphorylase activity associates with conditions like McArdle disease and Hers disease. McArdle disease involves a deficiency of PYGM leading to muscle pain and exercise intolerance. Conversely Hers disease results from deficient PYGL activity causing hypoglycemia and liver enlargement. In these conditions the malfunction of either PYGL or PYGM disrupts normal glycogen metabolism linking them to altered energy states and organ function.