Abcam, ab314437, Anti-PHC2 antibody [EPR27147-176] - ChIP Grade - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PHC2 antibody. Carrier free. Suitable for ChIP-seq, WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27147-176,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ChIP-seq, WB, IHC-P, IP, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for FC-intra human.

Product details:
ab314437 is the carrier-free version of
ab314436
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHC2 also known as Polyhomeotic Homolog 2 is a protein with a molecular mass around 100 kDa. It localizes mainly in the nucleus and expresses widely across different tissue types. The protein is a part of the Polycomb group (PcG) proteins which play key roles in chromatin remodeling and gene silencing. PHC2 contributes to forming stable gene repression essential for regulating development and maintaining cellular identity.
Biological function summary
PHC2 functions as a part of the Polycomb repressive complex 1 (PRC1). This complex is involved in modifying the structure of chromatin which helps maintain the repression of gene transcription. Through interaction with other PRC1 components PHC2 helps establish a compact chromatin state promoting histone modifications that lead to transcriptional silencing. This activity is fundamental for cellular processes like embryonic development and stem cell maintenance.
Pathways
PHC2 plays a role in the regulation of the Wnt signaling and Notch signaling pathways. These pathways are integral to controlling cell proliferation differentiation and apoptosis. Within these pathways PHC2 interacts with other proteins like BMI1 and RING1B which are also part of the PRC1 complex. These interactions ensure the precise control over gene expression needed for cellular homeostasis and proper developmental progression.
Aberrant PHC2 function is implicated in a range of conditions particularly cancer and neurodevelopmental disorders. Dysregulation of PHC2 can lead to misexpression of genes that regulate cell cycle and apoptosis contributing to oncogenesis. For instance PHC2 has connections with BMI1 in cancer pathways influencing tumor progression and resistance to apoptosis. Moreover mutations or irregular expression of PHC2 have been linked with neurodevelopmental disorders highlighting its importance in nervous system development and function.