Abcam, ab314529, Recombinant human CCR4 protein - Active (His tag)

Size: 20µg / 100µg
Recombinant human CCR4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 360 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level, suitable for WB, FuncS.
Key facts
Endotoxin level:< 1 EU/µg,
Expression system:Mammalian,
Tags:His tag C-Terminus,
Applications:WB, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA. AB314529 at 10 μg/mL can bind an Anti-CCR4 recombinant antibody, the EC50 is 362.3-630.8 ng/mL.,
Accession:P51679,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in water,
Storage buffer:pH: 7.4 - 8Constituents: 6% Trehalose, 0.14% PBS

Product details:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, -80°C, Appropriate long-term storage conditions--20°C, -80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCR4 also known as C-C chemokine receptor type 4 is a G protein-coupled receptor with the molecular mass of approximately 41 kDa. It is predominantly expressed on the surface of T-helper 2 cells regulatory T cells and certain dendritic cells. CCR4 functions by binding chemokines such as CCL17 and CCL22 facilitating chemotaxis of immune cells to sites of inflammation. Researchers often refer to antibodies targeting CCR4 like mogamulizumab when studying immune responses.
Biological function summary
CCR4 plays an important role in the trafficking of immune cells to inflammatory sites therefore regulating immune response and tolerance. It is part of the chemokine receptor family which is integral to the immune system's function. CCR4 helps maintain the balance between effector and regulatory T cells contributing to immunological homeostasis. Its involvement in migration and positioning of immune cells within lymphoid and peripheral tissues highlights its importance in maintaining immune surveillance.
Pathways
CCR4 significantly influences the chemokine signaling pathway which is pivotal in directing the movement and positioning of immune cells. This receptor interacts with key proteins like CCL17 and CCL22 to mediate cell chemotaxis in these pathways. Additionally CCR4 is associated with the T-cell receptor signaling pathway where it coordinates with other receptors and proteins to modulate immune responses.
Increased CCR4 expression has connections with various conditions such as adult T-cell leukemia/lymphoma and cutaneous T-cell lymphomas. In these diseases CCR4 assists tumor cells in evading the immune system fueling disease progression. Proteins like TARC (thymus and activation-regulated chemokine) and MDC (macrophage-derived chemokine) ligands for CCR4 often facilitate tumor cell migration and proliferation. Therapeutic approaches targeting CCR4 such as the anti-CCR4 antibody mogamulizumab have shown potential in treating these malignancies by disrupting these interactions.