Abcam, ab314690, Anti-Claudin18 antibody [43-14A]

Size: 20µL / 50µL
Anti-Claudin18 antibody [43-14A] (ab314690) is a mouse monoclonal antibody detecting Claudin18 in IHC-P . Suitable for Human .
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:43-14A,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Cross-reactivity has been checked and this antibody might cross with Claudin18.1 and Claudin18.2.The human gene CLDN18 has two protein isoforms, Claudin 18.1 and Claudin 18.2. The two isoforms differ within the N-terminal 69 amino acids. Claudin 18.1 expression is restricted to the lung. In contrast Claudin 18.2 expression is restricted to the stomach.

Product details:
Product Specifications
Anti-Claudin18 antibody [43-14A] (ab314690) is a mouse recombinant monoclonal antibody and is validated for use in in human samples.
Anti-Claudin18 antibody [43-14A] (ab314690) specifically detects Claudin18 (UniProt ID: P56856; Molecular weight: 28kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (50 µL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Claudin18 antibody [43-14A] (ab314690) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Related Products
Conjugation-ready, carrier free format available for antibody clone 43-14A -
ab314691
Target Information
Claudin-18 is a protein encoded by the CLDN18 gene. It belongs to the claudin family, which forms tight junction strands in epithelial cells. Claudin-18 plays a crucial role in maintaining the integrity of these cell junctions, which are essential for various physiological processes. Disruption of these junctions can lead to increased cancer invasiveness and metastasis. Claudin 18 is a key marker in oncology due to its role in cancer progression.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Claudin-18 often noted as Claudin18 or CLDN18 is a tight junction protein belonging to the Claudin family. The protein comes in different isoforms including Claudin 18.2 each having a distinct function and expression pattern. With an estimated molecular mass of approximately 24 kDa it is mainly expressed in epithelial cells of the stomach and specific lung epithelia. Researchers utilize it frequently in immunohistochemistry (IHC) studies often referred to as CLDN18 IHC for its role in maintaining cell-to-cell adhesion in these tissues.
Biological function summary
Claudin-18.2 integrates itself into a tight junction complex contributing to the selective permeability barrier in epithelial tissues. This barrier is essential for maintaining tissue homeostasis and protecting underlying tissue from various external factors. It plays a significant role in ion selectivity and barrier assurance of the epithelial cells-gastrointestinal site especially. The regulated expression and function of Claudin 18 are critical for effective epithelial barrier function.
Pathways
Claudin-18 is involved in pathways concerning epithelial morphogenesis and integrity. It aligns with occludins and other claudins to control paracellular transport and cellular integrity within these pathways. Through its association with proteins such as ZO-1 Claudin-18 ensures proper cell signaling and tight junction formation. Its regulation in epithelial cells contributes significantly to pathways that sustain cell structure and prevent uncontrolled leakage of solutes.
The altered expression of Claudin-18.2 is closely linked to gastric and lung cancers. Abnormal levels can disrupt the epithelial barrier contributing to tumor progression and metastasis. In gastric cancer altered Claudin-18 couples with overexpression of other proteins such as HER2 playing a role in the disease's aggressiveness. Researchers consider it a potential target for therapeutic interventions making Claudin-18.2 purchase or acquisition relevant for studies oriented toward cancer diagnostics and treatment.