Abcam, ab315067, pan-HMR GSK3 beta ELISA Kit

Size: 1 x 96Tests
pan-HMR GSK3 beta ELISA Kit is a single-wash 90-min Simplestep used to quantify pan-HMR GSK3 beta with a sensitivity of 34.77 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human, Mouse, Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 34.77 pg/mL,
Range:312.5 - 20000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
pan-HMR GSK3 beta SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GSK3 beta protein in Cell Lysate. Quantitate pan-HMR GSK3 beta with 34.77 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycogen synthase kinase 3 beta (GSK3B) also referred to as GSK-3 beta is a serine/threonine kinase with an approximate mass of 46 kDa. It is widely expressed in various tissues including the brain heart and liver highlighting its significance in diverse cellular processes. As a protein kinase GSK3B phosphorylates serine and threonine residues on its substrate proteins controlling their activity and stability.
Biological function summary
GSK3B participates in numerous cellular functions such as glycogen metabolism neuronal signaling and cellular adhesion. It forms part of a complex that is important for the regulation of various transcription factors and enzymes. GSK3B interacts with several cellular proteins to modulate their activity such as influencing the beta-catenin pathway that impacts cell growth and division. This regulation is essential for maintaining proper cellular function and response to external stimuli.
Pathways
GSK3B plays a significant role in the Wnt signaling pathway and the insulin signaling pathway. These pathways are essential for cellular development differentiation and energy metabolism. GSK3B interacts closely with proteins like beta-catenin in the Wnt pathway and regulates glycogen synthase in the insulin signaling pathway. Through these interactions it ensures precise control over cellular proliferation survival and glucose metabolism.
GSK3B has significant connections to disorders like Alzheimer's disease and type 2 diabetes. In Alzheimer's disease abnormal activity of GSK3B is linked to the hyperphosphorylation of tau protein which contributes to neurofibrillary tangles. In type 2 diabetes GSK3B's involvement in the insulin signaling pathway can impact insulin resistance. Its dysregulation also affects proteins like the tau protein in neurodegenerative disorders and insulin receptor substrate proteins in metabolic disorders.