Abcam, ab315209, Anti-Aurora A+B+C antibody [4A7]

Size: 100µL
Mouse Monoclonal Aurora A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AURKA.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4A7,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human AURKA.O14965

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7 - 8Preservative: 0.03% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aurora A B and C are serine-threonine kinases known for their key roles in cell cycle regulation. These kinases often referred to as Aurora kinases have molecular masses of approximately 46 kDa (Aurora A) 39 kDa (Aurora B) and 34 kDa (Aurora C). Aurora A is primarily expressed in centrosomes and spindle poles during mitosis. Aurora B localizes mainly to the inner centromere during early mitosis and relocates to the central spindle and midbody in later stages. Aurora C expression occurs predominantly in the testes and it also participates in meiotic cell division.
Biological function summary
The Aurora kinases are important in the regulation of mitosis. Aurora A facilitates centrosome maturation and bipolar spindle assembly while Aurora B functions as part of the chromosomal passenger complex that ensures accurate chromosome alignment and segregation. Aurora C although less studied appears to overlap in function with Aurora B particularly in meiosis. Together these kinases ensure the correct distribution of chromosomes to daughter cells maintaining genomic stability.
Pathways
Aurora kinases are integral components of the mitotic spindle assembly checkpoint and the cytokinesis process. They operate in synchrony with proteins like PLK1 and CENP-A ensuring precise temporal and spatial progression through cell division. In the p53 pathway Aurora A plays a role in cellular response to DNA damage influencing cell fate decisions. Their regulated activity helps maintain correct chromosome number and division fidelity during cell replication.
Incorrect regulation or overexpression of Aurora kinases associates with cancer and infertility. Aurora A and B are frequently upregulated in various cancers including breast and colorectal cancers where they may interact with oncogenic proteins like MYC and RAS. Aurora C mutations have been linked to cases of male infertility affecting processes important for successful spermatogenesis. These associations highlight the therapeutic potential of Aurora kinases as targets for drug development in cancer and reproductive health issues.