Abcam, ab315308, Human DRP1 ELISA Kit

Size: 1 x 96Tests
Human DRP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DRP1 with a sensitivity of 0.059 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.059 ng/mL,
Range:0.25 - 16 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human DRP1 SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DRP1 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human DRP1 with 0.059 ng/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab315065
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DRP1 protein also known as Dynamin-Related Protein 1 has a molecular weight of approximately 80 kDa. DRP1 is a GTPase that plays a central role in mitochondrial fission. The protein is widely expressed in many tissues with high levels in the brain heart and skeletal muscles. It functionally interacts with other proteins on the mitochondrial membrane to drive the division of mitochondria. As a mechanism DRP1 assembles into ring-like structures around constriction points on the outer mitochondrial membrane facilitating membrane scission.
Biological function summary
DRP1 regulates mitochondrial morphology by controlling mitochondrial division. This process is essential in maintaining the balance between mitochondrial fission and fusion which affects mitochondrial distribution and function. DRP1 also associates with protein complexes involved in this dynamic balance including MFF (mitochondrial fission factor) and FIS1 (fission 1 protein). Phosphorylation state of DRP1 influences its activity; different phosphorylation sites either activate or inhibit its function.
Pathways
The mechanical action of DRP1 integrates closely into the mitochondrial quality control and apoptosis pathways. Mitochondrial fission driven by DRP1 is necessary for the removal of damaged mitochondria through mitophagy. It also influences the apoptotic pathway where DRP1 translocates to mitochondria under pro-apoptotic signals often in interaction with proteins such as Bax and Bak that promote cytochrome c release. This relationship affects cell survival and energy homeostasis.
Improper regulation of DRP1 is linked to neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. Altered DRP1 activity or expression disrupts mitochondrial homeostasis contributing to neuronal cell death pathways. Connections with proteins such as Tau in Alzheimer's and Parkin in Parkinson's influence the progression of these disorders. Additionally DRP1's role in cardiac disorders highlights its importance in maintaining normal cardiac function through mitochondrial regulation.