Abcam, ab315313, Human CFHR3 ELISA Kit

Size: 1 x 96Tests
Human CFHR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR3 with a sensitivity of 8.523 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Serum, EDTA Plasma, Citrate plasma, Heparin Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.523 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CFHR3 SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CFHR3 protein in Serum, EDTA Plasma, Cit Plasma, and Hep Plasma. Quantitate Human CFHR3 with 8.523 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab315065
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement factor H-related protein 3 (CFHR3) also known as FHR-3 plays a role in modulating the complement system. This protein has a mass of approximately 42 kDa. CFHR3 is expressed in tissues like the liver and can be found circulating in the plasma. It belongs to the factor H protein family which participates in maintaining immune homeostasis by regulating complement activation.
Biological function summary
CFHR3 acts as an antagonist to complement factor H by competing for binding sites on target surfaces and complement components. It does not function as a standalone protein but interacts closely within a family that includes CFH and other complement factor H-related proteins. CFHR3 can potentially form complexes with similar proteins to help finely tune the activity of the complement system to prevent excessive immune response.
Pathways
CFHR3 is a part of the complement cascade system specifically the alternative pathway. It contributes to the negative regulation of this pathway which is important for preventing overactivation that could harm host tissues. The protein influences the binding of complement proteins like C3b an essential component in the complement cascade and interacts with other factor H-related proteins such as CFHR1 to modulate pathway activities.
CFHR3's regulatory role connects it to conditions like atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS mutations or deletions in CFHR3 sometimes in conjunction with CFHR1 can lead to uncontrolled complement activation contributing to disease progression. Similarly variations in CFHR3's sequence can affect AMD risk often in association with other complement regulatory proteins including CFH indicating its significant position in disease susceptibility through its regulatory function within the immune system.