Abcam, ab315320, Human C4a ELISA Kit

Size: 1 x 96Tests
Human C4a ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C4a with a sensitivity of 12.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Milk,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 12.7 pg/mL,
Range:37.5 - 1200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human C4a SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C4a protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, and Milk. Quantitate Human C4a with 4.245 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab315065
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement C4a also known as C4a anaphylatoxin is a component of the complement system with a molecular mass of approximately 9 kDa. This small protein originates from the proteolytic cleavage of complement component C4 which contributes to immune system activation. C4a is mainly expressed in the liver but can also be produced by other tissues during inflammation. It serves as a chemoattractant recruiting immune cells to sites of infection helping initiate a response against pathogens.
Biological function summary
Complement C4a acts as an anaphylatoxin participating in the complex immune system response. Anaphylatoxins like C4a C3a and C5a contribute to the inflammatory process by inducing smooth muscle contraction increasing vascular permeability and triggering histamine release from mast cells and basophils. C4a complements the activities of other complement components playing an important role in modulating inflammation and defense mechanisms in the body.
Pathways
The activation of C4a is an important step within the classical and lectin pathways of complement activation. These pathways involve the recognition and elimination of pathogens and unwanted cells. C4a along with its related proteins C4b and components C2 and C3 facilitates the formation of C3 convertase which amplifies the response and further clears pathogens. C4a also interacts with complement inhibitors which regulate these pathways to prevent excessive tissue damage during inflammation.
C4a anaphylatoxin has associations with autoimmune diseases and angioneoplasm. Changes in serum levels of C4a are observed in conditions like systemic lupus erythematosus where it may contribute to tissue damage. High C4a levels can correlate with disease activity and severity. Additionally C4a alongside other anaphylatoxins like C3a and C5a might play a role in angiogenesis influencing tumor progression and angiogenesis-related disorders serving as a potential biomarker for disease severity and prognosis.