Abcam, ab315374, Recombinant human GHRH protein

Size: 50µg / 100µg / 200µg / 1mg
Recombinant human GHRH protein is a Human Fragment protein, in the 19 to 108 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for WB, SDS-PAGE.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:His tag N-Terminus GST tag N-Terminus,
Applications:WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P01286,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in 10mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.,
Storage buffer:Preservative: 0.82% Proclin 300Constituents: 81.9% Trehalose, 16.2% PBS, 0.25% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.16% Sodium-N-Lauroylsarcosinate

Product details:
Reconstitute in 10mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1 month, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle, The product can be stored for up to 12 months

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The growth hormone-releasing hormone (GHRH) sometimes called somatoliberin is a hypothalamic peptide hormone with a molecular weight of approximately 5 kDa. It is expressed mainly in the arcuate nucleus of the hypothalamus. GHRH stimulates the synthesis and release of growth hormone (GH) from the anterior pituitary by binding to the GHRH receptor which is a G-protein-coupled receptor. This interaction prompts the pituitary to secrete growth hormone which is important for growth and metabolic processes in the body.
Biological function summary
Growth hormone-releasing hormone plays a critical role in the regulation of growth hormone which affects growth metabolism and cell repair. GHRH does not function as part of a larger complex but acts directly to influence the pituitary gland. The hormone's activities extend beyond growth regulation to impact metabolic processes including lipid and carbohydrate metabolism. Its release influences overall energy balance and body composition impacting muscle and fat tissue growth.
Pathways
GHRH is a major part of the growth hormone axis working in the hypothalamic-pituitary-somatotropic pathway. This axis involves the regulation of growth hormone release and subsequent insulin-like growth factor 1 (IGF-1) production in the liver which promotes growth and development. GHRH functions interact closely with other hypothalamic hormones like somatostatin which inhibits growth hormone release to maintain a balance of hormonal activities necessary for appropriate somatic growth.
The dysregulation of GHRH affects conditions such as acromegaly and growth hormone deficiency. Elevated levels of GHRH often due to tumors cause excessive growth hormone secretion resulting in acromegaly characterized by abnormal growth of hands feet and facial features. Reduced GHRH secretion leads to growth hormone deficiency impairing growth in children and influencing metabolism in adults. In these conditions alterations in the interactions among GHRH growth hormone and IGF-1 play an important role in the disease pathology.