Abcam, ab315814, Anti-68kDa Neurofilament/NF-L (C-term) antibody [EPR28784-63]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Human, Synthetic peptide - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28784-63,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), I-ELISA, IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 68kDa Neurofilament also known as NF-L (C-term) is a critical component of the cytoskeleton in neurons. It has a mass of approximately 68 kilodaltons and is expressed primarily in the central and peripheral nervous systems. As the smallest neurofilament protein NF-L plays a significant role in maintaining neuronal caliber by assembling into intermediate filaments. These networks of filaments provide structural support to neurons which is essential for proper nerve cell function and stability.
Biological function summary
68kDa Neurofilament integrates into a complex with other neurofilament proteins such as NF-M and NF-H to form mature neurofilament structures. These filaments are necessary for axonal transport influencing the distribution of organelles and proteins along the axon. The proper function of these complexes is essential for nerve conduction and integrity impacting axonal geometry directly which is critical for sustaining efficient neuronal signaling and plasticity in the nervous system.
Pathways
The 68kDa Neurofilament is central to the neurofilament signaling and axonal transport pathways. These pathways regulate axonal caliber and contribute to efficient neurotransmission. It interacts closely with other cytoskeletal proteins and motor proteins such as kinesin which supports the movement of cellular components within axons. This transport mechanism is important for normal neuronal function and adaptation to signals affecting the overall communication within neural networks.
Aberrations in the 68kDa Neurofilament are linked to neurodegenerative conditions like Amyotrophic Lateral Sclerosis (ALS) and Charcot-Marie-Tooth disease. In ALS misregulation of neurofilament proteins including NF-L leads to axonal transport disruptions and neuronal death. NF-L abnormalities also are associated with Charcot-Marie-Tooth disease where mutations in the gene encoding this protein disturb normal filament assembly and function contributing to peripheral neuropathy. Changes in neurofilament interactions with proteins like peripherin can exacerbate these disorders highlighting their role in disease progression and serving as potential targets for therapeutic interventions.