Abcam, ab315828, Anti-AAV8 + AAV3B antibody [HL2383-IgG3] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal AAV8 antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Adeno-associated virus 3B, Recombinant fragment - Adeno-associated virus - 8 samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:HL2383-IgG3,
Isotype:IgG3,
Carrier free:Yes,
Reacts with:Adeno-associated virus - 8, Adeno-associated virus 3B,
Applications:Dot, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab315828 is the carrier-free version of
ab315827
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adeno-associated virus serotype 8 (AAV8) and AAV3B are variants belonging to the parvovirus family known for their roles as vectors in gene therapy. These serotypes do not have a known mass as they are not proteins but viral capsids made up of proteins. AAV8 and AAV3B are expressed by recombinant technology and utilized as delivery vehicles in various tissues and cells. AAV8 is especially known for its efficiency in transducing liver muscle and retina while AAV3B shows strong affinity for human hepatocytes.
Biological function summary
AAV8 and AAV3B lack replicative properties in the absence of a helper virus making them a safe choice for gene delivery systems. Unlike proteins that are part of a complex these viruses exist independently but rely on the host cellular machinery for successful transduction. Through their distinct tissue tropisms these serotypes enable targeted delivery of genetic material which enhances therapeutic outcomes.
Pathways
AAV8 and AAV3B integrate into host cell processes by hijacking the endosomal trafficking and nuclear import pathways. They remain episomal avoiding integration into the host genome reducing the risk of insertional mutagenesis. AAV3B specifically associates with serotypes like AAV2 in receptor binding and entry pathways which can impact its efficacy and targeting strategy in therapeutic applications.
AAV8 has shown significant potential in liver-targeted therapies particularly in the treatment of hemophilia by delivering functional copies of the factor IX gene. AAV3B on the other hand is under investigation for its role in targeting hepatocellular carcinoma cells due to its affinity for hepatocytes. Collaboration with proteins like the erythroblastic leukemia viral oncogene homolog 2 (ErbB2) aids AAV3B's therapeutic strategies by honing its specificity to cancerous cells.