Abcam, ab315971, Anti-Pofut1 antibody [EPR26782-194]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal Pofut1 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26782-194,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein known as Pofut1 or protein O-fucosyltransferase 1 functions mechanically by adding fucose a sugar molecule to serine or threonine residues on proteins specifically within the epidermal growth factor-like (EGF-like) repeats of substrates. Pofut1 has a molecular weight of approximately 50 kDa. This protein is expressed in various tissues including heart liver and brain indicating its significant role across different biological systems. Researchers commonly refer to Pofut1 in studies focused on protein glycosylation processes.
Biological function summary
Pofut1 is responsible for the proper folding and functioning of specific proteins that undergo fucosylation. It does not technically form part of a multi-protein complex but interacts tightly with substrates it modifies. Through its enzymatic action Pofut1 influences essential biological activities in cell communication and signaling. This glycosyltransferase also plays a pivotal role in modulating Notch signaling a critical pathway in the development and tissue regeneration processes.
Pathways
The significance of Pofut1 is most notable within the Notch signaling pathway where it modifies the Notch receptor enabling effective ligand-receptor interactions. Dysregulation of this pathway can lead to critical developmental defects. Pofut1 also connects to other pathways such as the Fringe-mediated glycosylation pathway. In this context Pofut1-related proteins like Fringe glycosyltransferases operate to further influence Notch signaling outcomes through additional glycosylation processing.
The functionality of Pofut1 has links to specific syndromes and malignancies. For instance aberrations in its activity are associated with congenital heart defects due to disrupted Notch signaling affecting cardiac development. Additionally overexpression or mutations in Pofut1 relate to cancer as improper Notch signaling can lead to tumor progression. Relatedly proteins like Notch1 serve as intermediaries linking Pofut1 activity to such conditions thereby highlighting Pofut1's involvement in disease etiologies.