Abcam, ab316012, Anti-FYCO1 antibody [EPR28361-79]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal FYCO1 antibody. Suitable for WB, IHC-Fr, IP, IHC-P and reacts with Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28361-79,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-P, IP, WB, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for rat IHC-FR.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FYCO1 also known as FYVE and coiled-coil domain autophagy adaptor 1 is a protein that functions as an adaptor for the transport of autophagosomes along microtubules. It has a mass of approximately 147 kDa. FYCO1 is expressed in several tissues including the eye retina and cells involved in immune response such as macrophages. The protein contains various domains that facilitate its binding to phosphatidylinositol 3-phosphate on endosomal membranes and interaction with light chain 3 (LC3) proteins to tether autophagosomes to the cytoskeleton.
Biological function summary
FYCO1 plays an important role in the regulation of autophagy by promoting the movement of autophagosomes to the cell periphery where they can fuse with lysosomes for degradation of their cargo. It functions as part of the autophagic transport machinery and it interacts with Rab7 a small GTPase involved in endosome and autophagosome maturation. This interaction enhances FYCO1's ability to facilitate autophagic flux and maintain cellular homeostasis.
Pathways
FYCO1 participates in the autophagy and lysosomal degradation pathway which is important for maintaining cellular health by clearing damaged organelles and proteins. The protein's action relates to the mTOR signaling pathway a central modulator of cellular growth and metabolism impacting processes like cell proliferation and survival. FYCO1 works closely with proteins such as LC3 and Rab7 which are integral to autophagosome-lysosome fusion and cellular cleanup processes.
FYCO1 has been linked to cataracts particularly autosomal recessive congenital cataracts. Mutations in FYCO1 can lead to abnormal autophagosome transport disrupting lens transparency and causing cataract formation. The protein's dysfunction in autophagic pathways also ties it to a broader spectrum of neurological disorders though ongoing research continues to uncover these connections. Its interactions with proteins like Rab7 highlight a network of molecular activities critical to maintaining cellular organization and function.